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目的:优选平哮颗粒的水提醇沉工艺条件。方法:以岀固率、盐酸麻黄碱和芍药苷的总提取率为综合评价指标,采用正交试验法考察浸泡时间、加水量、煎煮次数、煎煮时间4个因素对提取工艺的影响;以盐酸麻黄碱和芍药苷的总转移率为考察指标,正交试验考察药液与药材比例、醇沉时间、醇沉体积分数对醇沉工艺的影响。结果:平哮颗粒的最佳水提工艺为加8倍量水煎煮3次,每次1 h;最佳醇沉工艺为水煎液浓缩至生药质量浓度2 g.mL-1,加乙醇使含醇体积分数达60%,搅匀,静置8 h。结论:该优选工艺可确保有效成分最大限度保留,可用于工业化生产。
Objective: To optimize the conditions of water extraction and alcohol precipitation of Pingxiao granules. Methods: The total extraction rate of ephedrine hydrochloride, ephedrine hydrochloride and paeoniflorin was taken as the comprehensive evaluation index. The effects of soaking time, adding amount of water, boiling times and decocting time on the extraction process were investigated by orthogonal test. Taking total transfer rate of ephedrine hydrochloride and paeoniflorin as indexes, orthogonal experiment was conducted to investigate the influence of the ratio of drug solution to herbs, alcohol precipitation time and alcohol sediment volume fraction on the alcohol precipitation process. Results: The optimum extraction process of Pingxiao granules was as follows: adding 8 times the amount of water and decocting for 3 times, each time being 1 h; the optimal alcohol precipitation process was decoction concentration to 2 g.mL-1 of crude drug concentration, adding ethanol The alcohol content of the volume fraction of 60%, stir, stand 8 h. Conclusion: This preferred process ensures maximum retention of active ingredients for industrial production.