Background:Angiogenesis is the formation of new blood vessels to supply nutrients to tumors.Vascular endothelial growth factor (VEGF) and cluster of differentiation 34 (CD34) are important signaling proteins involved in angiogenesis.Many studies have demonstrated that VEGF and CD34 are related to tumor progression.This study focused on the relationship between VEGF,CD34,and perioperative hemorrhage in patients with gastric cancer.Methods:To observe the relationship between VEGF and CD34,we tracked 112 patients with advanced gastric cancer for 5 years to assess factors related to hemorrhage,using immunohistochemistry.The results were subjected to statistical analysis using a 2 × 2 contingency table,logistic regression,and receiver operating characteristic (ROC) test.Results:The concentrations of VEGF and CD34 were critically correlated with perioperative hemorrhage and neural invasion in patients with gastric cancer (P ＜ 0.05).Expression of VEGF and CD34 was related (P ＜ 0.05,x2 =6.834).VEGF and CD34 co-expression strongly increased the risk of preoperative bleeding (area under the ROC curve ＞0.7,P ＜ 0.05).Conclusions:Expression of VEGF and CD34 was critically correlated with perioperative hemorrhage in gastric cancer patients.Co-expression of VEGF and CD34 could be an effective indicator for evaluating the risk ofperioperative bleeding in gastric cancer patients.