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目的：探讨血管紧张素转换酶抑制剂卡托普利和依那普利拉对心肌细胞内游离钙浓度的影响，并观察两药能否对抗缺血再灌注引起的细胞内钙超载作用。方法：Ｗｉｓｔａｒ大鼠心肌细胞培养７２小时后分为对照组、卡托普利组和依那普利拉组。药物于实验前３０分钟加入，终浓度为１０－５ｍｏｌ／Ｌ。Ｉｎｄｏ－１ＡＭ标记后，应用缺血再灌注损伤模型，用粘附式细胞仪连续观察缺血前、缺血及再灌注细胞内游离钙浓度（荧光比率）的变化。测试结果组间采用ｔ检验。结果：缺血前卡托普利组和依那普利拉组细胞内荧光比率均低于对照组（Ｐ＜０．０１～０．０５）；而缺血及再灌注阶段，对照组细胞内荧光比率持续升高，卡托普利组和依那普利拉组均保持相对平稳，显著低于对照组（Ｐ＜０．０１）。结论：血管紧张素转换酶抑制剂可以拮抗缺血再灌注心肌细胞内钙超载，对缺血再灌注心肌细胞具有明显的保护作用。
Objective: To investigate the effects of captopril and enalaprilat, an inhibitor of angiotensin converting enzyme, on intracellular free calcium concentration in cardiomyocytes and to observe whether the two drugs can counteract the intracellular calcium overload caused by ischemia / reperfusion. Methods: The myocardial cells of Wistar rats were divided into control group, captopril group and enalapril group after 72 hours. Drugs were added 30 minutes before the experiment, the final concentration of 10-5mol / L. After Indo-1AM labeling, the changes of intracellular free calcium concentration (fluorescence ratio) in ischemic and reperfused cells were observed continuously by using the adherent cytometer with ischemia-reperfusion injury model. Test results between groups using t test. Results: The intracerebral fluorescence ratios of captopril group and enalapril group before ischemia were lower than those of control group (P <0.01 ~ 0.05). In the phase of ischemia and reperfusion, Fluorescent ratio continued to increase, captopril group and enalaprilat group remained relatively stable, significantly lower than the control group (P <0.01). CONCLUSION: Angiotensin-converting enzyme inhibitors can antagonize calcium overload in myocardial cells during ischemia-reperfusion, and have a significant protective effect on myocardial cells after ischemia-reperfusion.