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目的制备pET-32a(+)-VP1蛋白的多克隆抗体。方法用纯化后的VP1蛋白分4次免疫兔子,颈动脉插管法取血,制得多克隆抗体。结果用ELISA法和Western blot鉴定多克隆抗体的效价得1?320000。该抗体可以用Western blot法检测出59 kD左右的VP1蛋白。结论成功制备高效价的JC病毒衣壳蛋白VP1多克隆抗体。
Objective To prepare a polyclonal antibody against pET-32a (+) - VP1. Methods Purified VP1 protein was used to immunize rabbits in 4 times and carotid arteries were cannulated to obtain polyclonal antibody. Results The titer of polyclonal antibody was determined by ELISA and Western blot. The antibody can be detected by Western blot about 59 kD VP1 protein. Conclusion The high titer polyclonal antibody of JC virus capsid protein VP1 was successfully prepared.