论文部分内容阅读
为揭示白杨派无性系对盐渍环境的适应能力,筛选速生耐盐碱优良无性系,本文采用盆栽试验方法,对8个白杨派无性系在不同浓度(0、30、60、90 mmol·L~(-1))NaHCO_3胁迫下的生长及生理代谢指标的变化进行了研究。结果表明:8个无性系的地上部分和根系生长均受到明显抑制,无性系26和89表现出较强的速生性;叶绿素含量均呈下降趋势,其中下降最小的为无性系26和89;随着NaHCO_3处理浓度的增加,8个无性系叶片脯氨酸含量持续增加,其中无性系26和89显著高于相同胁迫条件下其他无性系;NaHCO_3胁迫导致叶片细胞膜相对透性以及丙二醛(MDA)含量提高,但相同胁迫条件下无性系26、LM50、89细胞膜相对透性以及MDA含量均显著低于其他无性系。超氧化物岐化酶(SOD)活性呈先增大后减小的变化趋势,其中无性系89和26的酶活性始终高于其他无性系;在90 mmol·L~(-1) NaHCO_3处理下,苗高增长量、地径增长量与各生理指标之间的相关性均达显著或极显著相关,表明利用生长指标和生理指标对无性系耐盐性进行综合评价,更能反映NaHCO_3胁迫后的响应。利用主成分分析对8个无性系生长及生理代谢等参数进行综合评价,各无性系耐盐碱性由强到弱依次为26、89、LM50、150、Z3、27、1316、Y3。因此,初步认为无性系26和89具有显著耐盐碱性和速生性,可作为盐渍立地造林或抗逆育种的重要试验材料应用。
In order to reveal the climatic adaptability of Poplar clones to saline environment and to screen fast-growing saline-alkali-tolerant clones, the pot experiment was carried out to study the effects of clonal Poplar clones on the climatic conditions of eight Poplar clones at different concentrations (0, 30, 60 and 90 mmol·L ~ (-1)) NaHCO_3 stress and physiological and metabolic indicators were studied. The results showed that both the aerial parts and the root growth of eight clones were significantly inhibited, while the clones 26 and 89 showed strong fast-growing. Chlorophyll content tended to decrease. Among them, the lowest was 26 and 89 With the increase of NaHCO3 concentration, the proline contents of eight clones continued to increase, and the clones 26 and 89 were significantly higher than other clones under the same stress conditions; the relative permeability of the cell membrane and the content of malondialdehyde (MDA) ), But the relative membrane permeability and MDA content of clones 26, LM50 and 89 under the same stress were significantly lower than those of other clones. The activity of superoxide dismutase (SOD) firstly increased and then decreased, and the activities of clones 89 and 26 were always higher than those of other clones. Under the treatment of 90 mmol·L -1 NaHCO 3 , Seedling height growth, soil diameter growth and the correlation between the various physiological indicators were significant or very significant correlation, indicating that the use of growth and physiological indicators of clonal salt tolerance to conduct a comprehensive evaluation, better reflect the stress of NaHCO_3 the response to. Principal component analysis (PCA) was used to evaluate the parameters of growth and physiological metabolism in eight clones. The resistance to salinity and alkalinity of all clones was 26, 89, LM50, 150, Z3, 27, 1316 and Y3, respectively. Therefore, it is preliminarily believed that clones 26 and 89 have significant salt-salinity and fast-growing properties and can be used as important test materials for afforestation or anti-retrograde breeding in saline soils.