论文部分内容阅读
本文对L7811-85,L7212-85,L1210-86,P388-86 4个淋巴细胞白血病及S180-86和肝Ca-86肿瘤细胞株分别测定了细胞生长曲线、克隆形成效率和细胞DNA含量分布等参数。对其中L7811-85细胞株进行了较系统的细胞动力学研究,并应用~3H-TdR自杀试验微培养测定其克隆形成细胞的增殖时和合成时。结果表明:这6种细胞株均可在体外无刺激因子作用下良好生长。并可见随细胞传代数增加,细胞增殖加速,克隆形成率提高。用~3H-TdR自杀试验测出的克隆形成细胞增殖时与群体细胞倍增时相比,可反映其潜在倍增时。
In this study, cell growth curves, colony-forming efficiency, and cell DNA content distributions were measured in L7811-85, L7212-85, L1210-86, P388-86 4 lymphocytic leukemia and S180-86 and hepatic Ca-86 tumor cell lines. parameter. The systemic cell dynamics of L7811-85 cell line was studied, and the proliferation and synthesis of the cloned cells were determined by ~3H-TdR suicide microculture. The results showed that all six cell lines could grow well without stimulatory factors in vitro. It can be seen that as the number of passaged cells increases, cell proliferation accelerates and the colony formation rate increases. The proliferation of clone-forming cells, as measured by the ~3H-TdR suicide assay, may reflect the potential for doubling when compared to population doubling.