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目的探讨天麻素注射液对慢性缺血性大鼠生物钟基因表达的影响。方法 18只Sprague-Dawley雄性成年大鼠随机分为假手术组、模型对照组、天麻素组,各6只,采用双侧颈总动脉永久结扎法建立慢性脑缺血模型。造模21 d后,灌胃给药21 d,测量各组大鼠的体重;Western blotting和RT-PCR法分别检测大鼠松果体生物钟基因Clock、Bmal1和Per1的蛋白和mRNA表达水平。结果各组大鼠体重手术前期差异无统计学意义(P>0.05),术后7 d,模型对照组与假手术组差异有统计学意义(P<0.05)。给药结束后模型对照组较假手术组和天麻素组体重明显降低,假手术组与天麻素组差异无统计学意义(P>0.05)。慢性脑缺血下调大鼠松果体Clock基因、Per1基因表达,对Bmal1基因表达无影响。给予天麻素后,与模型对照组相比,Clock基因表达水平上调;与假手术组相比,Bmal1基因表达水平下降,Per1基因表达未见显著差异。结论天麻素注射液可能通过调节松果体生物钟基因的表达,达到治疗慢性脑缺血继发的睡眠障碍的目的。
Objective To investigate the effect of gastrodin injection on the gene expression of the circadian clock in chronic ischemic rats. Methods Eighteen Sprague-Dawley male adult rats were randomly divided into sham-operation group, model control group and gastrodin group, 6 rats in each group. Chronic cerebral ischemia model was established by permanent bilateral common carotid artery ligation. After 21 days, the body weight of rats in each group was measured by intragastric administration for 21 days. The protein and mRNA expression of circadian clock, Bmal1 and Per1 in pineal gland were detected by Western blotting and RT-PCR respectively. Results There was no significant difference in preoperative body weight between the two groups (P> 0.05). On the 7th day after operation, the difference between the model control group and the sham operation group was statistically significant (P <0.05). At the end of the administration, the body weight of the model control group was significantly lower than that of the sham operation group and the gastrodin group. There was no significant difference between the sham operation group and the gastrodin group (P> 0.05). Chronic cerebral ischemia reduced the expression of Clock gene and Per1 gene in rat pineal gland, but had no effect on Bmal1 gene expression. After administration of gastrodin, the expression of Clock gene was up-regulated compared with the model control group. Compared with the sham operation group, the expression of Bmal1 gene decreased and the expression of Per1 gene was not significantly different. Conclusion Gastrodin Injection may achieve the goal of treating sleep disorders secondary to chronic cerebral ischemia by regulating the expression of the circadian clock of pineal gland.