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目的:比较分离的柴胡-黄芩水提液各有效部位对脂多糖诱导的发热小鼠模型的退热影响。方法:柴胡-黄芩水提液经AB-8型大孔树脂柱净化,依次用水,20%,40%,60%和90%乙醇梯度洗脱,通过化学反应检识,合并组成相近的流份得到4个部位;观察各部位对脂多糖诱导的小鼠发热模型的退热作用。结果:柴胡-黄芩水提液经分离得到的4个部位分别为糖类(A)、皂苷类(B)、皂苷及黄酮苷类(C)、黄酮苷元及皂苷元(D);4个部位均具有不同程度的退热作用(P<0.05),其中以D部位的退热作用最好(P<0.01)。结论:柴胡-黄芩水提液退热作用涉及多个部位。
OBJECTIVE: To compare the antipyretic effects of the isolated fractions of Bupleurum chinense and Scutellaria baicalensis Georgi on fever model induced by lipopolysaccharide in mice. Methods: The aqueous extract of Bupleurum chinense and Scutellaria baicalensis was purified by AB-8 macroporous resin column and eluted with a gradient of 20%, 40%, 60% and 90% ethanol, followed by chemical reaction, The four parts were obtained. The antipyretic effects of different parts on mouse model of fever induced by lipopolysaccharide were observed. Results: The four fractions isolated from the extracts of Bupleurum chinense and Scutellaria baicalensis were carbohydrate (A), saponins (B), saponins and flavonoid glycosides (C), flavonoid aglycone and sapogenin (D); Each site had different levels of antipyretic effect (P <0.05), of which the best antipyretic activity in the D site (P <0.01). Conclusion: The antipyretic effect of Bupleurum chinense aqueous extract involves multiple sites.