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以3个大豆品种为材料,采用GUS瞬时表达的方法,对适合于大豆子叶节和胚尖转化的基因型进行筛选,结果表明:适合大豆子叶节转化的品种为东农50,适合胚尖转化的品种为黑农41。在子叶节转化系统中,摸索了农杆菌侵染浓度与侵染时间的最佳配比组合、乙酰丁香酮浓度和超声波辅助处理对大豆转化效率的影响。优化后的条件为:农杆菌侵染的浓度OD600=0.6,侵染时间30～40 min,乙酰丁香酮浓度200μmol.L-1,超声波辅助转化时间5 s。在上述条件下,成功获得了转基因植株,转化效率为2.3%。
Using three soybean cultivars as materials, the suitable genotypes of soybean cotyledonary node and embryo transformation were selected by GUS transient expression method. The results showed that the suitable soybean cultivar was Dongnong 50, which was suitable for embryo transformation The variety is black peasant 41. In the cotyledonary node transformation system, we explored the best combination of Agrobacterium tumefaciens infection concentration and infection time, the effect of acetosyringone concentration and ultrasonic assisted treatment on soybean transformation efficiency. The optimized conditions were Agrobacterium infection OD600 = 0.6, infection time 30-40 min, acetosyringone concentration 200 μmol·L-1 and ultrasound-assisted transformation time 5 s. Under the above conditions, the transgenic plants were successfully obtained and the transformation efficiency was 2.3%.