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目的研究大孔树脂富集纯化黄芪、三七、红参三药总皂苷的工艺参数,为“WNA制剂”二次开发的优化分离工艺提供参考。方法以香草醛-高氯酸法测定总皂苷含量,静态吸附法对D101、D301、AB-8三种吸附树脂进行筛选,进一步对工艺进行评价。结果用D101大孔吸附树脂,上样浓度为0.4g/ml,洗脱流速1ml/min,吸附流速1.0ml/min条件下,用3BV 70%乙醇洗脱,可得到较优的总皂苷含量。结论该技术方法简单可行,分离效果较好,可进一步开展中试研究,为实际生产提供技术参数。
Objective To study the technological parameters of macroporous resin enrichment and purification of total saponins from Radix Astragali, Panax notoginseng, and Radix Ginseng, and provide reference for the optimized separation process of secondary development of WNA Formulation. Methods The total saponin content was determined by the method of vanillin perchloric acid. The three adsorption resins, D101, D301 and AB-8, were screened by static adsorption method, and the process was further evaluated. Results The optimal total saponin content was obtained with D101 macroporous adsorption resin at a loading concentration of 0.4 g / ml, an elution flow rate of 1 ml / min and an adsorption flow rate of 1.0 ml / min using 3BV 70% ethanol. Conclusion The method is simple and feasible, and the separation effect is good. The pilot study can be further carried out to provide technical parameters for actual production.