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目的 提高蘑菇加工废水中剩余蘑菇多糖的利用,提高蘑菇多糖的附加经济值.方法 采用溶剂提取法提取蘑菇加工废水中的蘑菇多糖,并提纯、脱色、去蛋白,将处理过的粗多糖与锌元素配合形成复合多糖-锌(II),对最优化实验条件进行筛选.结果 提取最佳实验条件为在料液比为1∶40,浸提温度为90℃,微波功率60W,微波时间6min,预浸时间6h;脱色效果最佳实验条件为p H=3.0,时间60min,温度60℃,脱色剂用量为0.25g;蛋白去除最佳实验条件为震荡时间4min,静止时间30min,粗多糖:sevag为2:1,三氯甲烷:正丁醇为4∶1;复合多糖-锌(II)的最佳合成工艺为:配合反应的p H=3.0,反应时间60min,反应温度为50℃.结论 在提取条件为料液比为1∶40,浸提温度为90℃,微波功率60W,微波时间6min,预浸时间6h,脱色,去蛋白,蘑菇多糖的最高提取率为0.35％.在p H为3.0时,反应时间60min,反应温度为50℃时,复合多糖-锌(II)的配合效果最佳.“,”Objective To improve the additional economic value of mushroom polysaccharides, the polysaccharide extracted from mushroom wastewater was firstly cooperated with zinc ion. Methods The mushroom polysaccharides in mushroom wastewater were extracted, purified, decolorized and deproteinized. The crude polysaccharides were complexed with zinc to form complex polysaccharide-zinc ( II). And the optimization conditions were studied. Results The optimum extraction conditions were as followed the ration of material: liquid was 1: 40, the extraction temperature was 90 ℃, the microwave power was 60 W, the microwave time was 6 min and the pre-soaking time was 6 h. The best decolorizing effect was p H = 3, reaction time of 60 min, reaction temperature of 60℃, de-colorant dosage of 0. 25 g. The optimal conditions for protein removal were as followed shaking time was 4 min, resting time was 30 min, crude polysaccharides: sevag was 2: 1, chloroform: n-butanol was4: 1. The best synthesis process was as followed: the p H of the reaction was 3. 0, the reaction time was 60 min and the reaction temperature was 50℃. Conclusion The optimum conditions for the extraction of mushroom polysaccharide are 1: 40, 90℃, 60 W microwave power, 6 min microwave time and 6 h pre-soaking time. The maximum extraction rate of mushroom polysaccharide is 0. 35％. The complexing effect of polysaccharide-zinc ( II) was the best at p H value 3. 0, reaction time60 min and reaction temperature 50 ℃.