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应用杂交瘤技术,获得了8种CEA单克隆抗体(McAb),这8中McAb经~(125)I标记NCA,NFA,NCA(J)抑制试验,证明是针对CEA抗原决定簇的,取其中的三种 McAb混合后,其亲和常数为2×10~(-8)M,应用此混合McAb检测239例人次血清标本,85例正常人的CEA 平均值为3.6±9.1ng/ml,其中96％少于30mg/ml,而 5％位于30～50ng/ml。在 128 例肿瘤患者中,81例结直肠癌,阳性检出率为44％;22 例胃癌为55％;21例肺癌为57％;4例肝癌为25％。在91 例消化道肿瘤病理检测中阳性率达79％,正常胃对照18例皆为阴性。
Eight kinds of McAbs against CEA were obtained by hybridoma technology. The inhibition of NCA, NFA and NCA (J) in 125 McAbs by 8 McAbs proved to be against CEA epitopes. Of the three McAbs mixed, the affinity constant of 2 × 10 ~ (-8) M, using this mixed McAb 239 serum samples, 85 normal subjects CEA average of 3.6 ± 9.1ng / ml, of which 96% less than 30 mg / ml and 5% at 30-50 ng / ml. Of the 128 tumor patients, 81 were colorectal cancer with a positive rate of 44%; 22 with gastric cancer 55%; 21 with 57% with lung cancer and 25% with 4 cases with liver cancer. In 91 cases of gastrointestinal tumor pathology positive rate of 79%, 18 cases of normal gastric control were negative.