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目的探讨益气养阴消癥通络中药对糖尿病大鼠肾小球滤过屏障的影响。方法选择雄性SD大鼠110只,随机分为对照组、模型组、厄贝沙坦组和中药低、中、高剂量[5.29、10.58、21.16 g/(kg·d)]组。用左肾切除术加腹腔注射链脲佐菌素(STZ)制备糖尿病肾病模型,对照组只行左肾切除术。厄贝沙坦组大鼠给予厄贝沙坦15 mg/(kg·d)灌胃,益气养阴消癥通络中药低、中、高剂量组分别灌胃给药,对照组、模型组大鼠等剂量生理盐水灌胃,连续6周。实验结束时用PCR及Western blot印迹法测定肾组织CD2相关蛋白(CD2AP)及wilms’肿瘤抑制基因(WT-1)表达。结果与模型组比较,中药低、中、高剂量组和厄贝沙坦组CD2AP、WT-1表达显著增加(P<0.01)。结论益气养阴、消癥通络中药可保护肾小球滤过屏障外层裂孔膜和足细胞的结构与功能,延缓糖尿病肾病的发生发展。
Objective To investigate the effect of Yiqi Yangyin Xiaozhong Tongluo Chinese medicine on glomerular filtration barrier in diabetic rats. Methods 110 male SD rats were randomly divided into control group, model group, irbesartan group and low, medium and high dose [5.29,10.58,21.16 g / (kg · d)] groups. Diabetic nephropathy model was established by left nephrectomy plus intraperitoneal injection of streptozotocin (STZ), while the control group received only the left nephrectomy. The rats in irbesartan group were treated with irbesartan 15 mg / (kg · d) orally, while the rats in low, middle and high dose of Yiqiyangyinxiao Tongluo Chinese medicine group were given gavage, control group and model group Rats were given normal saline for 6 weeks. At the end of the experiment, the expressions of CD2AP and wilms’ tumor suppressor gene (WT-1) in renal tissues were determined by PCR and Western blotting. Results Compared with the model group, the expressions of CD2AP and WT-1 in low, medium and high dose group and irbesartan group increased significantly (P <0.01). Conclusions Chinese herbs for relieving qi and nourishing yin and eliminating collaterals can protect the structure and function of the outer perforation membrane and podocyte of glomerular filtration barrier and delay the occurrence and development of diabetic nephropathy.