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细胞内微黏度是描述细胞状态的重要物理参数,与物质转运和信号转导等一系列扩散控制的生理过程密切相关.构筑对细胞内微黏度响应灵敏的荧光探针是原位实时检测细胞状态的重要手段之一.我们设计含有D-π-A推拉电子结构N,N’-双水杨醛缩乙二胺类配体(N,N’-thiophene-3,4-bis-4’-(diethylamino)-salicylimine,Salen)的Zn~(2+)和Al~(3+)配合物,考察了其光物理性质,发现该类配合物具有对溶液黏度依赖的荧光增强性质.同时,相比于配合物ZnSalen,[AlSalen]~+Cl~-具有检测灵敏度高、荧光成像信号增强倍数高等优点.利用激光扫描共聚焦荧光成像和荧光寿命成像,[AlSalen]~+Cl~-同样表现出在培养温度降低或细胞自噬下的荧光增强和发光寿命延长等变化,显示其作为检测细胞内微黏度荧光探针的潜在应用价值.
Intracellular microviscosity is an important physical parameter to describe cell status, which is closely related to a series of diffusion-controlled physiological processes such as substance transport and signal transduction.Furthermore, constructing a fluorescent probe that is sensitive to intracellular microviscosity is a real-time detection of cell status One of the important ways we design a kind of N, N’-thiophene-3,4-bis-4’- (Zn 2+ and Al 3+) complexes of diethylamino-salicylimine and Salen were investigated for their photophysical properties and the fluorescence-enhanced properties of these complexes were found to be viscosity dependent. Compared with complex ZnSalen and [AlSalen] ~ + Cl ~ -, AlSalen ~ + Cl ~ - has the advantages of high detection sensitivity and high enhancement ratio of fluorescence imaging signal. [AlSalen] ~ + Cl ~ - also shows by laser scanning confocal fluorescence imaging and fluorescence lifetime imaging The results showed that it could be used as a potential fluorescent probe for detection of intracellular microviscosity by reducing the temperature of culture or increasing the fluorescence intensity and prolonging the luminescent life under autophagy.