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目的:在微弧氧化钛表面构建壳聚糖(CS)/antimiR-138涂层并研究其生物学性能。方法:用CS包裹antimiR-138形成CS/antimiR-138载体颗粒,通过硅烷偶联的方法将其加载至经微弧氧化(MAO)处理的钛表面。再将钛试件分为MAO表面加载CS/antimiR-138组、加载CS/antimiR-control组、加载硅烷偶联处理组(MAO-SGC)和未经MAO处理组(MAO)(n=5),用接触角测量仪测量其表面水接触角;SEM观察其表面微形貌;CCK-8分析其生物相容性;RT-PCR检测其对MSCs内miR-138及成骨相关基因表达的影响。结果:各组钛表面的水接触角为MAO组0.05);RT-PCR结果表明,MAO-CS/antimiR-138组表面细胞内miR-138的表达量明显低于其他3组(P<0.05),各成骨相关基因的表达量明显高于其他3组(P<0.05)。结论:在微弧氧化钛表面构建CS/antimiR-138涂层,生物相容性优良,可促进MSCs的成骨分化。
OBJECTIVE: To construct chitosan (CS) / antimiR-138 coating on micro-arc titanium oxide surface and study its biological properties. METHODS: CS / antimiR-138 vector particles were coated with antimiR-138 by CS and loaded onto the MAO-treated titanium surface by silane coupling. Then, the titanium specimens were divided into the MAO-loaded CS / antimiR-138 group, the CS / antimiR-control group, the MAO-SGC loaded group and the MAO-treated group (n = 5) , The surface water contact angle was measured by the contact angle measuring instrument; the surface micro-topography was observed by SEM; the biocompatibility was analyzed by CCK-8; the expression of miR-138 and osteogenesis-related genes in MSCs was detected by RT-PCR . Results: The water contact angle of each group of titanium surface was MAO group 0.05). The results of RT-PCR showed that the expression of miR-138 in MAO-CS / antimiR-138 group was significantly lower than that in the other three groups (P <0.05), and the expression of each osteogenesis- Other three groups (P <0.05). CONCLUSION: CS / antimiR-138 coating is constructed on the surface of micro-arc titanium oxide and has good biocompatibility. It can promote the osteogenic differentiation of MSCs.