为深入调查中华按蚊的种内变异,作者利用上海中华按蚊实验室品系的四龄幼虫制备,观察了大量唾腺染色体标本。方法是:在5％醋酸中解剖出唾腺,再用50％醋酸固定1～2,然后用2％地衣红(由50％醋酸和50％乳酸配制)染色,高倍光学显微镜观察,油镜下照相。在百余张标本中终于发现了几个由染色体倒位形成的杂合体。在制备良好的标本中开始不联会的始 To further investigate the intraspecific variation of Anopheles sinensis, the authors used a fourth-instar larvae of the laboratory strain of Anopheles sinensis to prepare a large number of salivary gland chromosome specimens. The method is: Salivary glands are dissected in 5% acetic acid and fixed with 1 to 2 of 50% acetic acid, then stained with 2% lichen red (formulated with 50% acetic acid and 50% lactic acid), observed under high magnification light microscope, Camera. In hundreds of specimens finally found several inversions formed by chromosome hybrids. Beginning of a non-union session in well-prepared specimens