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目的:探讨乳腺浸润性导管癌(IDC)中乳腺癌扩增性抗原1(AIB1)和增殖细胞核抗原(Ki67)蛋白的表达及临床意义。方法:选择2012年6月到2014年6月在我院经病理检查确诊为IDC患者的组织石蜡标本160例,采用链霉素-生物素(SP)免疫组化法检测标本中AIB1和Ki67蛋白的表达,多因素Logistic回归分析二者与IDC临床病理学特征的相关性。结果:AIB1、Ki67的阳性表达率分别为75.63%和80.63%。AIB1、Ki67的表达与淋巴结转移、组织学分级及TNM分期存在相关性(P<0.05),且随组织学分级和TNM分期的增高,阳性表达率逐渐增高(P<0.05),Ki67的表达水平随肿瘤变大,阳性率逐渐增加(P<0.05)。淋巴结阳性组AIB1、Ki67的阳性表达率显著高于淋巴结阴性组(P<0.05)。多因素Logistic回归分析显示,AIB1、Ki67的阳性表达是淋巴结转移、病理组织学分级及TNM分期的危险因素(P<0.05)。结论:在IDC组织中AIB1和Ki67的阳性表达均增高,二者与IDC临床病理学特征有密切关系。
Objective: To investigate the expression and clinical significance of AIB1 and Ki67 in invasive ductal carcinoma of the breast (IDC). Methods: Sixty-six paraffin-embedded specimens from IDC patients confirmed by pathological examination in our hospital from June 2012 to June 2014 were selected for detection of AIB1 and Ki67 protein by streptavidin-biotin (SP) immunohistochemistry The multivariate logistic regression analysis was used to analyze the correlation between the two and clinicopathological characteristics of IDC. Results: The positive rates of AIB1 and Ki67 were 75.63% and 80.63% respectively. The expression of AIB1 and Ki67 was correlated with lymph node metastasis, histological grade and TNM stage (P <0.05), and the positive expression rates of Ki67 and Ki67 were significantly increased with the histological grade and TNM stage (P <0.05) With the tumor became larger, the positive rate increased (P <0.05). The positive expression rates of AIB1 and Ki67 in lymph node positive group were significantly higher than those in negative node group (P <0.05). Multivariate logistic regression analysis showed that the positive expression of AIB1 and Ki67 were the risk factors of lymph node metastasis, histopathological grade and TNM staging (P <0.05). Conclusion: The positive expression of AIB1 and Ki67 in IDC tissues are both elevated, which are closely related to the clinicopathological features of IDC.