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目的 探讨p5 3基因突变和p5 3蛋白表达与食管癌临床病理的关系。方法 应用银染PCR -SS CP方法和免疫组织化学方法检测了食管癌组织中p5 3基因突变和蛋白的表达。以切缘正常组织为对照。结果 4 7例食管癌标本中 ,p5 3基因第 5～ 8外显子出现点突变者 2 0例 ,其突变率为 4 2 .6 % ,生存期大于 5年组和生存期小于 5年组 ,p5 3突变率分别为 32 .1%和 5 7.9% ,差异无显著性 (χ2 =3.0 71,P >0 .0 5 ) ;2 2例伴有淋巴结转移的原发癌中 13例出现了突变 ,阳性率为 5 9.1% ,与无淋巴结转移组相比 ,有显著性差异 (P <0 .0 5 )。p5 3蛋白表达者 2 9例 ,其表达率为 6 1.7% ,生存期大于 5年组与生存期小于 5年组相比 ,p5 3蛋白阳性表达率分别为 5 0 .0 %和 78.9% ,具有显著性差异 (P <0 .0 5 ) ,有淋巴结转移组和无淋巴结转移组相比 ,p5 3蛋白表达阳性率分别为 77.3%和 4 8.0 % ,也具有显著性差异 (P <0 .0 5 )。结论 p5 3基因突变和蛋白表达与食管癌的转移密切相关 ,且是一个判断预后的有用指标
Objective To investigate the relationship between the mutation of p5 3 gene and the expression of p5 3 and the clinicopathological features of esophageal cancer. Methods The p5 3 gene mutation and protein expression in esophageal cancer tissues were detected by silver staining PCR-SSCP and immunohistochemistry. The margins of normal tissue as a control. Results Twenty-four cases of p5 3 gene mutation in exon 5 ~ 8 were found in 47 specimens of esophageal cancer, the mutation rate was 42.6%, the survival time was longer than 5 years and the survival time was less than 5 years , the mutation rate of p5 3 was 32.1% and 5 7.9%, respectively, with no significant difference (χ2 = 3.071, P> 0.05). Thirteen cases of primary cancer with lymph node metastasis appeared in 22 cases The mutation rate was 51.9%, which was significantly different from that in the group without lymph node metastasis (P <0.05). The positive rate of p5 3 protein was 50.0% and 78.9% respectively in 29 cases with p5 3 protein expression of 67%, with a survival time of more than 5 years and less than 5 years survival time. (P <0.05). The positive rates of p5 3 protein expression in lymph node metastasis group and non-lymph node metastasis group were 77.3% and 48.0%, respectively. There was also significant difference (P <0. 0 5). Conclusion The mutation and protein expression of p5 3 are closely related to the metastasis of esophageal cancer, and it is a useful index to judge the prognosis