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观察TLR9在实验性变态反应性神经炎(EAN)大鼠的坐骨神经、脾脏、外周血、淋巴结的动态表达,探讨TLR9与EAN的发病关系,为临床寻找新的治疗自身免疫性疾病的策略奠定理论基础。将36只Lewis大鼠随机分为抗原注射组(EAN组:n=4,4,4,4),完全弗氏佐剂组(CFA组:n=4,4,4,4),生理盐水对照组(NS组:n=4),EAN组用抗原乳剂(100μg P0 180~199,100μl完全弗氏佐剂,100μl生理盐水)进行免疫,观察免疫后大鼠的发病情况和组织病理学改变。分别在第7天、第16天、第24天,第33天处死动物,取坐骨神经根、脾脏、外周血和淋巴结,制作病理切片行HE染色和Weil染色观察其病理改变,利用RT-PCR检测TLR9的mRNA表达水平。结果:EAN大鼠在发病16 d时症状达高峰,第33天时好转,TLR9 mRNA整个实验过程中一直呈上升趋势,前后各时间点相比具有统计学意义(P<0.05),与对照组相比有显著差异。结论:TLR9在EAN的起始及效应阶段有着重要作用。
To observe the dynamic expression of TLR9 in the sciatic nerve, spleen, peripheral blood and lymph nodes of rats with experimental allergic neuritis (EAN), to explore the relationship between TLR9 and EAN, to lay a theory for finding a new strategy for the treatment of autoimmune diseases basis. 36 Lewis rats were randomly divided into antigen injection group (EAN group: n = 4,4,4,4), complete Freund’s adjuvant group (CFA group: n = 4,4,4,4), normal saline Control group (NS group: n = 4). EAN group was immunized with antigen emulsion (100μg P0 180 ~ 199, 100μl Complete Freund’s adjuvant, 100μl saline) to observe the incidence and histopathological changes of rats after immunization. The animals were sacrificed on the 7th day, the 16th day, the 24th day and the 33rd day, respectively. The sciatic nerve roots, spleen, peripheral blood and lymph nodes were obtained and pathological changes were made by HE staining and Weil staining. TLR9 mRNA expression levels. Results: The symptoms of EAN rats peaked on the 16th day and improved on the 33rd day. The TLR9 mRNA level had been increasing throughout the experiment. There was a significant difference (P <0.05) between the time point and the control group Than the significant difference. Conclusion: TLR9 plays an important role in the initiation and effector stage of EAN.