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目的：探讨羟苯氨酮（ｏｘｙｐｈｅｎａｍ ｏｎｅ ， Ｏｘｙ） 的钙增敏机理。方法：从牛心肌组织中提取ｃ Ｔｎ Ｃ，采用荧光探针标记法观察 Ｏｘｙ 对ｃ Ｔｎ Ｃ 与 Ｃａ２ ＋ 亲和力的影响，并和已知的钙增敏剂 Ｍ Ｃ Ｉ１５４ 、磺甲唑（ｓｕｌｍａｚｏｌｅ ， Ｓｕｌ） 作比较。结果： Ｏｘｙ 显著增加ｃ Ｔｎ Ｃ 和 Ｃａ２ ＋ 的亲和力，且呈剂量与效应相关。而５０ μｍ ｏｌ· Ｌ－ １ Ｍ Ｃ Ｉ１５４ 或 Ｓｕｌ 对ｃ Ｔｎ Ｃ 和 Ｃａ２ ＋ 亲和力曲线均无显著影响。结论： Ｏｘｙ 的钙增敏机理可能与直接增加ｃ Ｔｎ Ｃ 与 Ｃａ２ ＋ 的亲和力有关。
Objective: To investigate the calcium sensitizing mechanism of oxyphenamone (Oxy). Methods: Extract cTn C from the bovine myocardium and observe the effect of Oxy on the affinity of cTn C and Ca2 + by fluorescent probe labeling method. And it is related to the known calcium sensitizers M C I-154, sulmazole, Sul). RESULTS: Oxy significantly increased the affinity of cTn C and Ca2 +, and was dose-response-related. However, 50 μmol · L-1 M C I-154 or Sul had no significant effect on cTn C and Ca 2+ affinity curves. Conclusion: The mechanism of calcium sensitization of Oxy may be related to the direct increase of the affinity of cTn C and Ca2 +.