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目的研究力达霉素对人急性髓性白血病细胞(HL-60)增殖和诱导分化的作用。方法用不同浓度的力达霉素处理HL-60细胞,通过MTT法检测力达霉素对细胞的增殖抑制作用;利用瑞氏-姬姆萨染色法观察力达霉素对细胞形态的影响;通过硝基蓝四氮(NBT)还原比色法测定力达霉素对HL-60细胞的诱导分化作用。结果不同浓度力达霉素处理细胞后,HL-60细胞的增殖能力明显受到抑制。当力达霉素的作用浓度超过100μmol.L-1时,抑制率可高达99%,并且抑制作用呈现时间依赖性和剂量依赖性的特点。W right-G iem sa染色结果显示,力达霉素作用HL-60细胞后,细胞在形态学方面发生很大变化。同时,力达霉素能够提高细胞的NBT还原能力。1 nmol.L-1和0.1 nmol.L-1浓度的力达霉素可以使HL-60细胞的NBT还原率分别提高到39.2%和54.5%;0.01 nmol.L-1和0.001 nmol.L-1浓度的力达霉素则使阳性细胞的百分率增加到78.6%和89.9%,与对照组相比,差异具有显著性(P<0.01)。结论力达霉素具有较强的抑制HL-60细胞增殖的能力,同时,低浓度力达霉素能使HL-60细胞形态发生改变,NBT还原阳性率升高,诱导细胞发生分化,其作用机制有待于进一步深入研究。
Objective To investigate the effect of lidamycin on the proliferation and differentiation of human acute myeloid leukemia cells (HL-60). Methods HL-60 cells were treated with different concentrations of lidamycin, and the inhibitory effect of lidamycin on the proliferation of HL-60 cells was detected by MTT assay. The effect of lidamycin on cell morphology was observed by Wright-Giemsa staining. The effect of lidamycin on HL-60 cells was determined by NBT reduction colorimetry. Results After HL-60 cells were treated with different concentrations of lipids, the proliferation of HL-60 cells was significantly inhibited. When the concentration of lidamycin over 100μmol.L-1, the inhibition rate can be as high as 99%, and the inhibition time-dependent and dose-dependent characteristics. The Wright-G iem sa staining results showed that the morphology of the cells changed greatly after HL-60 cells were treated with lidamycin. At the same time, lidamycin can improve NBT reduction ability of cells. Lidamycin at concentrations of 1 nmol.L-1 and 0.1 nmol.L-1 increased the NBT reduction rates of HL-60 cells to 39.2% and 54.5%, respectively. 0.01 nmol.L-1 and 0.001 nmol.L- 1 concentration of lidamycin increased the percentage of positive cells to 78.6% and 89.9%, which was significantly different from the control group (P <0.01). Conclusion Lidamycin has a strong inhibitory effect on the proliferation of HL-60 cells. At the same time, the low concentration of doxycycline can change the morphology of HL-60 cells, increase the positive rate of NBT, and induce the differentiation of HL-60 cells Mechanism needs to be further studied.