论文部分内容阅读
采用聚合酶链反应（ＰＣＲ），分别对２０例骨结核患者标本用两种方法（ＰＣＲ法及结核杆菌培养法）检查的阳性率分别为：培养法２０％，ＰＣＲ６５％，经统计学处理二种方法有显著性差别（Ｐ＜０．０１），ＰＣＲ阳性率显著高于培养法，而且２天即可报告，显著缩短了报告时间。３０例非骨结核患者标本两种方法均为阴性。盲法结核杆菌和对照菌ＰＣＲ结果表明具有较高的持异性。只要标本中有结核杆菌ＤＮＡ即可扩增其特异的ＤＮＡ片断，即使经福尔马林固定２～４周的标本也可检测，并不影响结果，这对于骨结核的进一步鉴别诊断都具有一定的意义。ＰＣＲ具有快速、特异、灵敏等优点，是在基因水平上诊断骨结核的一种新技术，是对各种骨结核病诊断和鉴别诊断的一种实用而可靠的检测手段。
Polymerase chain reaction (PCR) were used to detect the positive rates of 20 cases of patients with bone tuberculosis by two methods (PCR and Mycobacterium tuberculosis) respectively: culture method 20%, PCR 65%, after statistical processing two There was a significant difference between the methods (P <0.01). The PCR positive rate was significantly higher than that of the culture method, and could be reported in two days, which significantly shortened the reporting time. 30 samples of non-tuberculosis patients were negative in both methods. PCR results of Blight Mycobacterium tuberculosis and control bacteria showed high heterosexuality. As long as the specimens of Mycobacterium tuberculosis DNA can amplify its specific DNA fragments, even after formalin 2 to 4 weeks of fixed specimens can be detected, does not affect the results, which for the further differential diagnosis of bone tuberculosis have a certain Meaning. PCR is rapid, specific and sensitive. It is a new technique for diagnosing bone tuberculosis at the gene level and is a practical and reliable method for the diagnosis and differential diagnosis of all kinds of bone tuberculosis.