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目的研究肺癌转移相关转录子1(MALAT1)在亚慢性氯化镉(Cd Cl2)染毒大鼠模型肝肾组织中的表达情况,探讨MALAT1与镉致肝肾损伤过程中的关系。方法在已建立的亚慢性Cd Cl2染毒大鼠模型中,采用实时荧光定量反转录PCR(QRT-PCR)技术检测肝肾组织中MALAT1表达情况,分析MALAT1与测定的肝功能生化指标(ALT、AST)、肾功能生化指标(BUN、SCR)、相关基因(FOXC2、BCL-2、BAX、TGF、STAT、EGFR)及肝肾镉含量的相关性。结果与各自的对照组相比,大鼠肝肾组织中MALAT1表达水平均呈不同程度的升高,并有随染毒剂量的增高而升高的趋势,高剂量组表达量分别为(2.63±1.31)和(2.73±0.99),均为对照组的2.46倍,差异均有统计学意义(均P<0.05)。肝脏组织中MALAT1表达与血清中ALT含量存在相关性(r=0.459,P<0.05);肾脏组织中MALAT1表达与肾镉(r=0.427),BUN(r=0.531),SCR(r=0.400)含量及EGFR表达水平(r=0.466)均存在相关关系(均P<0.05)。结论 MALAT1在亚慢性镉染毒大鼠肝肾组织中表达水平增高,且与肝肾功能指标含量存在相关关系。肺癌转移相关转录子1可能在镉致肝肾损伤过程中发挥作用。
Objective To investigate the expression of MALAT1 in liver and kidney of rats exposed to sub-chronic cadmium chloride (CdCl2) and to explore the relationship between MALAT1 and cadmium-induced liver and kidney injury. Methods The expression of MALAT1 in liver and kidney tissues was detected by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (PCR-RT-PCR) in the established sub-chronic CdCl 2 -induced rat model. The relationship between MALAT1 and the measured biochemical markers of liver function , AST, BUN, SCR, FOXC2, BCL-2, BAX, TGF, STAT, EGFR) and liver and kidney cadmium content were determined. Results Compared with the control group, the expression of MALAT1 in rat liver and kidney increased to some extent, and increased with the increase of the dosage. The expression of MALAT1 in the high dose group was (2.63 ± 1.31) and (2.73 ± 0.99), both of which were 2.46 times of the control group, the difference was statistically significant (all P <0.05). The expression of MALAT1 in liver tissue correlated with the level of ALT in serum (r = 0.459, P <0.05). The expression of MALAT1 in renal tissues was significantly correlated with the levels of renal cadmium (r = 0.427), BUN Content and EGFR expression level (r = 0.466) (P <0.05). Conclusions The expression of MALAT1 in liver and kidney of rats exposed to subchronic cadmium is increased, and it is correlated with the index of liver and kidney function. Lung metastasis-associated transcript 1 may play a role in cadmium-induced liver and kidney injury.