Nitrogenous heterocyclic compounds are key pollutants in coking wastewater,however,the functional potential of microbial communities for biodegradation of such contaminants during biological treatment is still elusive.Herein,a high throughput functional gene array (GeoChip 5.0)in combination with Illumina HiSeq2500 sequencing was used to compare and characterize the microbial community functional structure in a long run (500 days) bench scale bioreactor treating coking wastewater,with a control system treating synthetic wastewater.Despite the inhibitory toxic pollutants,GeoChip 5.0 detected almost all key functional gene (average 61,940 genes) categories in the coking wastewater sludge.With higher abundance,aromatic ring cleavage dioxygenase genes including multi ring1,2diox;one ring2,3diox;catechol represented significant functional potential for degradation of aromatic pollutants which was further confirmed by Illumina HiSeq2500 analysis results.Response ratio analysis revealed that three nitrogenous compound degrading genes-nbzA (nitro-aromatics),tdnB (aniline),and scnABC (thiocyanate) were unique for coking wastewater treatment,which might be strong cause to increase ammonia level during the aerobic process.Additionally,HliSeq2500 elucidated carbozole and isoquinoline degradation genes in the system.These findings expanded our understanding on functional potential of microbial communities to remove organic nitrogenous pollutants;hence it will be useful in optimization strategies for biological treatment of coking wastewater.