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目的:观察大鼠颈动脉损伤后炎症因子的变化及应用罗格列酮干预后对其表达的影响。方法:将SD大鼠随机分为对照组、球囊损伤组、罗格列酮组,每组12只。对照组0.9%氯化钠溶液灌胃4d后假手术,术后0.9%氯化钠溶液灌胃13d;球囊损伤组0.9%氯化钠溶液灌胃4d后行左侧颈总动脉球囊损伤,术后0.9%氯化钠溶液灌胃13d;罗格列酮组用罗格列酮灌胃4d后行球囊损伤,术后罗格列酮灌胃13d。3组术后14d取左侧颈总动脉应用Realtime RT-PCR检测损伤血管组织中白细胞介素(interleukin,IL)-2、IL-10、IL-17A水平,应用Western Blot检测核因子κB(nuclear factor-kappa B,NF-κB)水平。损伤血管行苏木精-伊红染色,观察内膜变化。结果:①罗格列酮组IL-2、IL-17A表达水平低于球囊损伤组但高于对照组(P<0.05)。罗格列酮组IL-10表达高于球囊损伤组和对照组(P<0.05)。②罗格列酮组NF-κB水平低于球囊损伤组但高于对照组(P<0.05)。③罗格列酮组内膜面积及内膜面积/中膜面积较球囊损伤组减小但高于对照组(P<0.05)。结论:罗格列酮通过NF-κB调节IL-2、IL-10及IL-17AmRNA表达,调节炎症因子的平衡,抑制损伤血管的炎症反应,减轻损伤血管的狭窄。
OBJECTIVE: To observe the changes of inflammatory factors after carotid artery injury in rats and the effect of rosiglitazone on their expression. Methods: SD rats were randomly divided into control group, balloon injury group and rosiglitazone group, with 12 rats in each group. 0.9% sodium chloride solution in control group was administered 4 days after gavage, and 0.9% sodium chloride solution was administered intragastrically for 13 days. Balloon injury group received 0.9% sodium chloride solution for 4 days, then left common carotid artery balloon injury , 0.9% sodium chloride solution was administered intragastrically for 13 days after operation. Rosiglitazone group was treated with rosiglitazone 4 days after balloon injury, and rosiglitazone was administered intragastrically for 13 days. The left common carotid arteries were harvested at 3 days after operation in 3 groups. The levels of interleukin (IL) -2, IL-10 and IL-17A were detected by Realtime RT-PCR. The levels of nuclear factor κB factor-kappa B, NF-κB) levels. Injured blood vessels were hematoxylin-eosin staining to observe changes in the endometrium. Results: ①The expression of IL-2 and IL-17A in rosiglitazone group was lower than that in balloon injury group (P <0.05). The expression of IL-10 in rosiglitazone group was higher than that in balloon injury group and control group (P <0.05). ② The level of NF-κB in rosiglitazone group was lower than that in balloon injury group (P <0.05). Rosiglitazone group intimal area and intimal area / medial membrane area than the balloon injury group but higher than the control group (P <0.05). Conclusion: Rosiglitazone regulates the expression of IL-2, IL-10 and IL-17A mRNA through NF-κB, regulates the balance of inflammatory cytokines, inhibits the inflammatory reaction of injured blood vessels and reduces the stenosis of damaged blood vessels.