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①目的探讨人乳头瘤病毒１６型和１８型（ＨＰＶ１６和ＨＰＶ１８）感染与上皮性卵巢癌的关系。②方法选取１９９１～１９９３年存档的上皮性卵巢癌石蜡包埋组织４２例，良性上皮性卵巢肿瘤石蜡包埋组织２６例，正常卵巢石蜡包埋组织２０例。用聚合酶链反应（ＰＣＲ）技术测定了上述组织内ＨＰＶ１６－ＤＮＡ和ＨＰＶ１８－ＤＮＡ．③结果卵巢癌组织９例（２１．４％）ＨＰＶ１６－ＤＮＡ阳性，１５例（３５．７％）ＨＰＶ１８－ＤＮＡ阳性，二者比较差异无显著性（χ２＝２．１０，Ｐ＞０．０５）；良性卵巢肿瘤和正常卵巢组织中，ＨＰＶ１６－ＤＮＡ和ＨＰＶ１８－ＤＮＡ均为阴性；ＨＰＶ１８的感染与卵巢癌组织学分级有关（χ２＝４．６９，Ｐ＜０．０５），ＨＰＶ１６和ＨＰＶ１８的感染与卵巢癌临床分期及组织学分类均无关（χ２＝０．００３，０．６５０，Ｐ均＞０．０５）。④结论ＨＰＶ１６和ＨＰＶ１８的感染可能与上皮性卵巢癌发生有关。
Objective To investigate the relationship between human papillomavirus type 16 and 18 (HPV16 and HPV18) infection and epithelial ovarian cancer. Methods Forty-two cases of epithelial ovarian cancer paraffin-embedded tissues, 26 cases of benign epithelial ovarian tumors and 20 cases of normal ovarian paraffin-embedded tissues were selected from 1991 to 1993. The HPV16-DNA and HPV18-DNA in the above tissues were determined by polymerase chain reaction (PCR) technique. Results HPV16 DNA was positive in 9 cases (21.4%) of ovarian cancer tissues and HPV18-DNA in 15 cases (35.7%), the difference was insignificant (χ2 = 2.10, P> 0.05 ); HPV16-DNA and HPV18-DNA were all negative in benign ovarian tumors and normal ovarian tissues; HPV18 infection was associated with histological grade of ovarian cancer (χ2 = 4.69, P <0.05) Infection was not associated with clinical stage and histological classification of ovarian cancer (χ2 = 0.003, 0.650, P> 0.05). ④ Conclusion The infection of HPV16 and HPV18 may be related to the occurrence of epithelial ovarian cancer.