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以枸杞为原料,一步水热法制备得到具有强烈荧光的碳量子点(CQDs),并基于Cu(Ⅱ)与CQDs形成无辐射复合体,构建了“关-开”型荧光探针用于D-青霉胺(D-PA)的选择性高灵敏检测.在CQDs溶液中加入Cu(Ⅱ)离子后,CQDs的荧光发生淬灭,体系的荧光信号处于“关闭”状态.在D-PA存在下,由于D-PA与Cu(Ⅱ)具有更强的结合力,形成比CQDs-Cu(Ⅱ)复合体更稳定的络合物,将Cu(Ⅱ)从CQDs表面移除下来,使CQDs的荧光得到恢复,体系的荧光信号呈“打开”状态.实验探讨了Cu(Ⅱ)对CQDs荧光淬灭的机理,考察了各种反应条件.在优化的条件下,CQDs探针的荧光恢复程度与D-PA的浓度在0.5~80μmol L~(-1)范围呈良好的线性关系,检出限为0.15μmol L~(-1).应用于人血清中D-PA的检测,回收率为96.7%~105.0%,相对标准偏差小于3.1%,表明该法可应用于人体内D-青霉胺药物的检测和药代动力学研究.
Using Wolfberry as raw material, one-step hydrothermal method was used to prepare carbon quantum dots (CQDs) with strong fluorescence, and a non-radiative complex was formed based on Cu (Ⅱ) and CQDs. D-penicillamine (D-PA) selective and sensitive detection.CQDs in the solution after the addition of Cu (Ⅱ) ions, CQDs fluorescence quenching, the fluorescence signal system is in the “off” state in In the presence of D-PA, Cu (Ⅱ) is removed from the surface of CQDs due to the stronger binding of D-PA to Cu (Ⅱ) to form a more stable complex than CQDs-Cu , The fluorescence of CQDs was recovered and the fluorescence signal of the system was “on”. The mechanism of fluorescence quenching of CQDs by Cu (Ⅱ) was investigated and various reaction conditions were investigated. Under the optimal conditions, The fluorescence intensity of the needle was linear with the concentration of D-PA in the range of 0.5 ~ 80μmol L -1 with a detection limit of 0.15μmol L -1. The detection and recovery rates were 96.7% -105.0% with relative standard deviations less than 3.1%, indicating that this method can be applied to the detection and pharmacokinetic study of D-penicillamine in human.