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目的探讨癫癎发作过程中细胞凋亡与一氧化氮合酶(NOS)、核因子-B(NF-B)的关系。方法采用戊四氮(PTZ)致癎大鼠模型,检测癫癎发作后神经细胞凋亡、NOS和NF-B的变化。结果细胞凋亡率在癫癎发作后6 h开始升高,至24 h达到高峰,48 h下降,与对照组比较差异有显著性(P<0.05)。NOS在癫癎发作后1 h、24 h有2个分泌高峰,与对照组比较差异有显著性(P<0.05);对照组大鼠海马没有NF-B核转位细胞,而致癎后NF-B核转位细胞显著上调,24 h达高峰(P<0.01)。结论PTZ致癎大鼠模型中NOS早期可能参与癫癎发生,后期可能通过诱导NF-B产生而参与了神经细胞的凋亡。
Objective To investigate the relationship between apoptosis and the expression of nitric oxide synthase (NOS) and nuclear factor-B (NF-B) in epileptic seizures. Methods Rat model of pentylenetetrazole (PTZ) was used to detect the changes of neuronal apoptosis, NOS and NF-B after epileptic seizures. Results The rate of apoptosis increased at 6 h after onset of epilepsy, peaked at 24 h and decreased at 48 h, which was significantly different from the control group (P <0.05). There was a peak of 2 secretion at 1 h and 24 h after epileptic seizure in NOS group (P <0.05). There was no NF-B nuclear translocation in hippocampus of rats in control group, B nuclear translocation cells were significantly up-regulated, peaked at 24 h (P <0.01). Conclusion The early stage of NOS may play an important role in the development of epilepsy in PTZ induced rat model. The latter stage may be involved in the apoptosis of neural cells by inducing the production of NF-B.