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为观察集落刺激因子（ＣＳＦ）对慢性粒细胞白血病（ＣＭＬ）病理克隆增殖的影响，采用单抗免疫磁珠二步分离法，从ＣＭＬ患者骨髓标本分离出带有恶性克隆标记的ＣＤ３４＋ＨＬＡＤＲ＋细胞，并在体外观察干细胞因子（ＳＣＦ）、白细胞介素３（ＩＬ３）和促红细胞生成素（Ｅｐｏ）联合应用对细胞增殖的影响。结果：ＣＳＦ存在体系与无ＣＳＦ体系相比ＣＭＬ病理克隆消失延迟１４ｄ（Ｐ＜０．０５）。结果提示：ＣＳＦ具有延长ＣＭＬ病理细胞在体外培养存活期限的作用。
To observe the effect of colony-stimulating factor (CSF) on the proliferation of pathologic clones of chronic myelogenous leukemia (CML), a monoclonal antibody-immunomagnetic two-step isolation method was used to isolate CD34+HLA-DR+ with malignant cloned markers from bone marrow samples of CML patients. Cells and the effects of combined use of stem cell factor (SCF), interleukin-3 (IL-3) and erythropoietin (Epo) on cell proliferation were observed in vitro. RESULTS: The disappearance of CML pathological clones was delayed by 14 days compared with the CSF-free system (P<0.05). The results suggest that CSF has the effect of prolonging the survival period of CML pathological cells cultured in vitro.