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与胚胎体外培养有关的一些化学物质,如液体石蜡,CO_2、乙醇和水等,对小鼠早期胚胎发育有明显的影响。有些厂家生产的液体石蜡对胚胎有毒害作用,这种毒害作用与其比重和所含的硝基萘杂质无关;有的国产液体石蜡可用于早期胚胎培养。培养液中混入乙醇对胚胎发育危害很大,浓度达到0.8％时,胚胎完全不发育;浓度低于0.05％时,则无明显影响。酒精厂生产的CO_2粗制品,乙醇含量约为0.0013％,完全适合于做胚胎培养工作,用三蒸水配制的培养液进行胚胎体外培养,可获得良好效果。
Some chemicals related to embryo culture in vitro, such as liquid paraffin, CO 2, ethanol and water, have obvious effects on early embryonic development in mice. Some manufacturers of liquid paraffin toxic effects on the embryo, the poisoning effect and its contained in the proportion of nitronaphthalene impurities unrelated; some domestic liquid paraffin can be used for early embryo culture. Embedding of ethanol in culture solution is very harmful to embryo development. At the concentration of 0.8%, the embryo does not develop at all. When the concentration is lower than 0.05%, it has no obvious effect. Alcohol factory production of CO_2 crude products, ethanol content of about 0.0013%, fully suitable for embryo culture work, with three distilled water culture medium for embryo in vitro culture, get good results.