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目的了解医院多耐药鲍氏不动杆菌中转座子、插入序列遗传标记的存在情况。方法收集医院2008年3-11月患者标本中分离的多耐药鲍氏不动杆菌共20株,采用聚合酶链反应(PCR)的方法分析tnpU、tnp513、ISaba1等转座子、插入序列遗传标记及ISaba1-ADC连锁检测。结果 20株多耐药鲍氏不动杆菌中检出tnpU、tnp513转座子遗传标记各15株,检出率均为75.0%;ISaba1插入序列遗传标记17株,检出率为85.0%;ISaba1-ADC连锁检测17株,检出率为85.0%。结论该组多耐药鲍氏不动杆菌对β-内酰胺类、氨基糖苷类等药物呈多药耐药,可能与菌株携带转座子、插入序列等可移动遗传元件相关。
Objective To understand the existence of multidrug resistant Acinetobacter baumannii transposon in hospitals and genetic markers of inserted sequences. Methods A total of 20 strains of multidrug-resistant Acinetobacter baumannii isolated in the hospital from March to November in 2008 were collected. The transposons of tnpU, tnp513 and ISaba1 were analyzed by polymerase chain reaction (PCR) Marker and ISaba1-ADC chain test. Results Among 20 strains of multidrug-resistant Acinetobacter baumannii, 15 were found for tnpU and 15 for tnp513 transposon, respectively. The detection rate was 75.0%. ISaba1 was inserted into 17 genetic markers with the detection rate of 85.0% Seventeen strains were detected by ADC, the detection rate was 85.0%. Conclusion The multi-drug resistant Acinetobacter baumannii was multidrug-resistant to β-lactams, aminoglycosides and other drugs, which may be related to the portable genetic elements such as transposons and insertions.