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本文就两种正常细胞及五种恶性肿瘤细胞的自发荧光发射光谱和结合血卟啉衍生物(HpD)后的荧光发射光谱作了初步研究。结果表明,两类细胞的自发荧光发射光谱基本一致,正常细胞结合HpD后的荧光发射光谱与其自发荧光发射光谱类似,而五种恶性肿瘤细胞与HpD结合后的荧光发射光谱有特征性的波峰。
In this paper, preliminary studies have been conducted on the autofluorescence emission spectra of two kinds of normal cells and five malignant tumor cells and the fluorescence emission spectra after combining with hematoporphyrin derivatives (HpD). The results showed that the autofluorescence emission spectra of the two types of cells were basically the same. The fluorescence emission spectrum of normal cells after HpD binding was similar to that of their autofluorescence emission spectra, and the fluorescence emission spectra of the five malignant tumor cells combined with HpD had characteristic peaks.