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目的对疑似手足口病的住院患儿结合临床症状应用分子生物学方法进行实验室检测,及早确定治疗方案与预防策略,防止暴发流行。方法对疑似患儿进行流行病学调查,采集肛拭纸、咽拭纸、疱疹液及脑脊液,提取RNA,运用3对特异性引物进行PCR扩增,对阳性病例的性别、年龄和流行月份等指标进行统计学分析。结果 80例疑似手足口病患儿经RT-PCR检测,肠道病毒通用引物阳性的有64例(80%),其中EV71引物阳性的39例(48.75%),COX-A16引物阳性的19例(23.75%),EV71与COXA16引物同时阳性的有6例(7.5%),患儿性别差异无统计学意义(P>0.05),全年病例主要分布在5月份(18.75%)。无死亡病例。结论对手足口病疑似患儿进行病原学检测,能为临床治疗及制定预防措施提供科学依据。
Objective To detect the clinical symptoms of children with suspected hand-foot-mouth disease by molecular biology and to determine the treatment plan and prevention strategy as early as possible so as to prevent the outbreak from occurring. Methods Epidemiological investigation of suspected children was carried out. Anal swabs, pharyngeal swabs, herpes fluid and cerebrospinal fluid were collected and RNA was extracted. Three pairs of primers were used for PCR amplification. The gender, age and prevalence month of the positive cases Indicators for statistical analysis. Results 80 cases of suspected hand-foot-mouth disease were detected by RT-PCR and 64 cases (80%) were positive with enterovirus universal primers, of which 39 cases (48.75%) were EV71 primer positive and 19 cases were COX-A16 primer positive (23.75%). There were 6 cases (7.5%) of positive EV71 and COXA16 primers. There was no significant difference in sex between children and adolescents (P> 0.05). The cases were mainly distributed in May (18.75%). No deaths. Conclusions The etiological detection of suspected foot and mouth disease in children can provide a scientific basis for clinical treatment and preventive measures.