[Objective] To develop a high performance liquid chromatography(HPLC) method to simultaneously determine contents of three phenolic compounds including gallic acid,methyl gallate and 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose in the fruits of Toona sinensis(A.Juss.) Roem..[Method] The chromatographic separation was performed on a Hypersil C18 column(150 mm×6.0 mm,5 μm) with mobile phase of methanol and 0.1% formic acid in gradient elution whose flow rate was 1.0 ml/min.The temperature of sample manager was set at 30 ℃,and samples were detected at 280 nm and quantified by external standard method.[Result] The linear ranges of gallic acid,methyl gallate and 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose were respectively 0.048-0.48 μg,0.018-0.18 μg and 0.022-0.22 μg with correlation coefficient of more than or equal to 0.999 8.The average recoveries of 3 phenolic compounds were respectively 101.6%,99.5% and 99.1% with RSD of less than or equal to 2.9%.[Conclusion] The developed HPLC method was simple,sensitive and accurate with good separation,which was available for determining contents of phenolic compounds in the fruits of Toona sinensis(A.Juss.) Roem.. [Objective] To develop a high performance liquid chromatography (HPLC) method to determine the contents of three phenolic compounds including gallic acid, methyl gallate and 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose in the fruits of Toona sinensis (A. Juss.) Roem .. [Method] The chromatographic separation was performed on a Hypersil C18 column (150 mm × 6.0 mm, 5 μm) with mobile phase of methanol and 0.1% formic acid in gradient elution whose flow rate was 1.0 ml / min. The temperature of sample manager was set at 30 ° C and samples were detected at 280 nm and quantified by external standard method. [Result] The linear ranges of gallic acid, methyl gallate and 1, 2,3,4,6-penta-O-galloyl-β-D-glucopyranose were respectively 0.048-0.48 μg, 0.018-0.18 μg and 0.022-0.22 μg with correlation coefficient of more than or equal to 0.999 8. The average recoveries of 3 phenolic compounds were respectively 101.6%, 99.5% and 99.1% with RSD of less than or equal to 2.9%. Conclusion The developed HP LC method was simple, sensitive and accurate with good separation, which was available for determining contents of phenolic compounds in the fruits of Toona sinensis (A.Juss.) Roem.