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紫花苜蓿高频再生体系的建立有助于促进其遗传转化的研究。以陇东、阿尔冈金以及甘农4号紫花苜蓿为实验材料,比较了体细胞胚胎和丛生芽再生途径对于紫花苜蓿再生周期及频率的影响。并通过利用不同外植体、不同植物生长调节剂配比,以实现对这两种再生途径的进一步优化。实验结果表明:在三个苜蓿品种中,甘农4号的再生效果较好,下胚轴的再生频率较高,在愈伤诱导阶段添加2 mg/L 2,4-D、1 mg/L KT,胚状体诱导阶段添加1 mg/L KT、0.5 mg/L NAA可以达到较好的再生效果;在培养基中添加1 mg/L 6-BA、1 mg/L TDZ,则直接诱导丛生芽的效果最好;对比发现,丛生芽再生途径显示出较强的优越性,其再生时间仅10周,是紫花苜蓿再生体系建立的一种可选方式。
The establishment of high-frequency regeneration system of alfalfa helps to promote its genetic transformation. Longdong, Algonquin and Gannon No.4 alfalfa were used as experimental materials to compare the effects of somatic embryo regeneration and clustered bud regeneration on the regeneration cycle and frequency of alfalfa. And through the use of different explants, different plant growth regulator ratio in order to achieve further optimization of these two regeneration pathways. The experimental results showed that among the three alfalfa varieties, Gannong 4 had better regeneration and higher frequency of hypocotyls regeneration. During the callus induction stage, 2 mg / L 2,4-D and 1 mg / L KT and embryoid body induction stage, 1 mg / L KT and 0.5 mg / L NAA could achieve better regeneration effect. When 1 mg / L 6-BA and 1 mg / L TDZ were added into the medium, the tufts were directly induced Buds have the best effect. Compared with the regenerated alfalfa regeneration system, the regenerative pathway of the regenerated clump of buds shows a strong superiority and regeneration time is only 10 weeks.