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将逆行追踪剂荧光金分别注入大鼠一侧臂旁区、延髓尾侧腹外侧区和颈髓第四节段 ,观察到延髓背角浅层 ( 、 层 )向上述部位均有投射 , 层外侧部的投射神经元多于 层内侧部。臂旁区接受双侧延髓背角浅层的投射 ,但以同侧为主 ;延髓尾侧腹外侧区和第 4颈髓接受同侧延髓背角浅层神经元的投射但延髓背角浅层向第 4颈髓投射的神经元数量较少。结合免疫荧光组织化学研究表明 , 层向臂旁区和延髓尾侧腹外侧区投射的神经元部分呈 calbindin-D2 8k样阳性 ,而未见呈 parvalbumin样阳性者。向第 4颈髓投射的 层神经元数量很少 ,未见有呈 calbindin-D2 8K和 parvalbumin样阳性者。本研究结果进一步支持 层有传出投射的观点 ,也提示 层的 calbindin-D2 8K样和 parvalbumin样阳性神经元可能分别属于不同的细胞亚群。
The retrograde tracing agent fluorescent gold were injected into the rat parabrachial region, medulla oblongata lateral ventricle and the fourth segment of the cervical cord, the medullary dorsal horn was observed to the above site projection, the lateral layer The Ministry projects more neurons than the inner part of the floor. The parabrachia region received the projection of the superficial medullary dorsal horn but mainly of the same side; the medulla oblongata lateral vena caudalis and the fourth cervical spinal cord received the projection of the superficial neurons of the ipsilateral medullary dorsal horn but the medullary dorsal horn superficial The number of neurons projecting to the fourth spinal cord is small. Combined with immunofluorescence histochemical studies showed that the neurons projecting to the parabrachial and medulla oblongata regions showed calbindin-D2 8k-like positive, but no parvalbumin-like positive. There was only a small number of neurons projecting to the fourth spinal cord. No calbindin-D2 8K and parvalbumin-like positive cells were seen. The results of this study further support the idea that the layer has projected projection and also suggest that the calbindin-D2 8K-like and parvalbumin-like neurons in the layer may belong to different cell subpopulations, respectively.