论文部分内容阅读
分别以人型支原体（ＭＨ）、解脲支原体（ＵＵ）三个血清型（ＳＵ１、ＳＵ４与ＳＵ８）全ＤＮＡ与等体积光敏生物素混合，用特种紫外光照射制备成探针。在分离培养的基础上，用上述全ＤＮＡ光敏生物素探针，对８２例非淋菌尿道炎病人标本作了检测，结果分离培养ＭＨ２２例，ＵＵ５５例；探针法检出ＭＨ２０例，ＵＵ５３例，两方法ＭＨ、ＵＵ阳性标本均有重叠，提示ＵＵ感染率较ＭＨ感染率高，且ＭＨ感染多数伴随在ＵＵ感染中。三型ＵＵ探针检测结果表明某一单型ＵＵ探针不能代替分离培养检测出所有型ＵＵ。
The total DNA of three serotypes (SU1, SU4 and SU8) of human mycoplasma (MH) and UU (UU) were mixed with an equal volume of biotin, respectively, and prepared into a probe by special UV irradiation. On the basis of isolation and culture, 82 cases of non-gonococcal urethritis were detected with the above-mentioned all-DNA biotin probe. Results MH22 and UU55 cases were isolated and cultured, 20 cases of MHU and 53 cases of UU were detected by probe method, Two methods of MH, UU positive specimens overlap, suggesting that UU infection rate is higher than the MH infection, and the majority of MH infection with UU infection. Three type UU probe test results show that a single type UU probe can not replace the culture detected all types of UU.