自1985年Fenech和Morley建立了胞浆分裂阻滞微核法(即CB微核法)以来,CB微核法已广泛应用于人外周血淋巴细胞中,以检测辐射诱导的微核。1989年我们在Fenech和Morley的CB法基础上建立了人微量全血法。目前,我们又着重从Cyt B浓度、Cyt B加入时间和培养时间三方面,探讨了狗外周血淋巴细胞CB微核法。 CB Micronucleus has been widely used in human peripheral blood lymphocytes to detect radiation-induced micronuclei since the establishment of the cytosolic block micronuclei (ie, CB micronucleus method) by Fenech and Morley in 1985. In 1989, we established the human trace blood method based on the CB method of Fenech and Morley. At present, we also focused on the CB micronucleus assay of peripheral blood lymphocytes of dogs from three aspects of the concentration of Cyt B, the addition time of Cyt B and the culture time.