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目的建立一种发病机制、病理改变与临床接近且造模周期短的房颤小动物模型,并探讨白介素-17A(interleukin-17A,IL-17A)、C反应蛋白(C reactive protein,CRP)在房颤发生中的变化。方法 20只健康SD大鼠采用随机数字表法分为房颤组和阴性对照组,房颤组经食道快速心房起搏后,观察房颤诱发的情况,并记录房颤诱发前后心电图的改变。酶联免疫吸附法测定血浆中IL-17A、CRP的浓度,Masson三色染色法观察心房组织纤维化程度的改变。结果房颤组大鼠经食道快速心房起搏后,均造模成功,总诱发率为96%,持续时间为(45.52±60.88)s。与对照组比较,房颤组大鼠心率显著加快,QRS间期及QT间期均显著延长(P<0.05)。房颤组血浆中IL-17A、CRP水平较对照组均显著升高,且随房颤发生次数的增多浓度有上升趋势,房颤组心房纤维化程度较对照组严重,胶原容积分数显著增大,差异均有统计学意义(P<0.05)。结论经食道快速心房起搏方法构建房颤大鼠模型成功率高、重复性好、周期性短,是较理想的造模方法之一;IL-17A、CRP可能在房颤发生发展中起一定作用。
OBJECTIVE: To establish a small animal model of atrial fibrillation (AF) with pathogenesis, pathological changes and clinical modeling, and to investigate the relationship between interleukin-17A (IL-17A) and C reactive protein Changes in atrial fibrillation. Methods Twenty healthy SD rats were randomly divided into atrial fibrillation group and control group. The atrial fibrillation group was induced by atrial fibrillation after rapid atrial pacing. Atrial fibrillation was recorded before and after atrial fibrillation. The concentrations of IL-17A and CRP in plasma were determined by enzyme-linked immunosorbent assay (ELISA), and the changes of atrial fibrosis were observed by Masson’s trichrome staining. Results After atrial fibrillation, rats were auricularly parenchymal transesophageal atrial pacing. The total induction rate was 96% and the duration was (45.52 ± 60.88) s. Compared with the control group, the heart rate of the rats in atrial fibrillation group was significantly increased, and the QRS interval and QT interval were significantly prolonged (P <0.05). The plasma levels of IL-17A and CRP in AF group were significantly higher than those in control group, and increased with the increase of the number of atrial fibrillation. The degree of atrial fibrosis in AF group was more serious than that in control group, and the collagen volume fraction was significantly increased , The differences were statistically significant (P <0.05). Conclusion The rapid atrial fibrillation in transesophageal atrial pacing rat model has high success rate, good repeatability and short periodicity. It is an ideal method to make model of atrial fibrillation. IL-17A and CRP may play a certain role in the development of atrial fibrillation effect.