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本文结果表明，血管活性肠肽（ＶＩＰ）可刺激人结肠腺癌ＨＴ２９细胞产生ｃＡＭＰ，呈剂量依赖关系，ＶＩＰ与异丁基甲基黄嘌呤（ＩＢＭＸ）联用刺激作用更强。较低剂量的ＶＩＰ或双丁酰环磷酸腺苷（ｄｂ－ｃＡＭＰ）单独能刺激细胞增殖，而高剂量ＶＩＰ或ｄｂ－ｃＡＭＰ，以及ＷＰ联用ＩＢＭＸ则抑制ＨＴ２９细胞增殖。流式细胞仪分析表明，ＶＩＰ联用ＩＢＭＸ或ｄｂ－ｃＡＭＰ单独可延长细胞Ｇ０／Ｇ１期。
The results of this study indicate that vasoactive intestinal peptide (VIP) can stimulate the production of cAMP in human colon adenocarcinoma HT29 cells in a dose-dependent manner, and the combination of VIP and isobutylmethylxanthine (IBMX) has a stronger stimulatory effect. Lower doses of VIP or db-cAMP alone stimulated cell proliferation, whereas high doses of VIP or db-cAMP and WP in combination with IBMX inhibited HT29 cell proliferation. Flow cytometry analysis showed that VIP combined with IBMX or db-cAMP alone could prolong the G0/G1 phase of cells.