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在大鼠小肠缺血再灌注 (IIR)模型上 ,研究磷脂酶A2 (PLA2 )在IIR所致肺损伤发病过程中的作用 ,观察大黄对PLA2 影响 ,探讨大黄防治肠源性肺损伤的机理。方法 SD大鼠随机分为肠缺血再灌注组、假手术组、大黄治疗组和安慰剂组。以12 5I标记小牛血清白蛋白 (BSA)肺摄取指数作为评价肺损伤的指标 ,以髓过氧化物酶 (MPO)作为评价多聚核白细胞(PMN)在组织中聚集的指标 ,分别测定各组动物不同时间血清、肺及小肠组织PLA2 活性。结果 大黄可明显抑制再灌注导致的肺MPO活性升高 (P <0 0 1)及肠缺血期和再灌注期血清、肺及小肠组织PLA2 活性升高 (P <0 0 5或P <0 0 1) ,降低肺毛细血管通透性 (P <0 0 1)。结论 早期应用大黄能明显防治大鼠IIR所致的肺损伤 ,这种作用至少部分是通过抑制PLA2 活性实现的
To investigate the role of phospholipase A2 (PLA2) in the pathogenesis of lung injury induced by IIR on the rat intestinal ischemia-reperfusion (IIR) model, to observe the effect of rhubarb on PLA2 and to explore the mechanism of rhubarb in preventing intestinal-induced lung injury. Methods SD rats were randomly divided into intestinal ischemia reperfusion group, sham operation group, rhubarb treatment group and placebo group. The lung uptake index of 12 5I-labeled bovine serum albumin (BSA) was used as an index to evaluate lung injury, and myeloperoxidase (MPO) was used as an index to evaluate the aggregation of polynuclear leukocytes (PMN) in tissues. Group animals had PLA2 activity in serum, lung, and small intestine at different times. RESULTS: Rhubarb could significantly inhibit the increase of lung MPO activity (P < 0.01) and the activity of PLA2 in serum, lung and small intestine during ischemia and reperfusion (P <0 05 or P <0). 0 1) decreased pulmonary capillary permeability (P < 0 01). Conclusion Early application of rhubarb can obviously prevent lung injury induced by IIR in rats, and this effect is at least partially achieved by inhibiting PLA2 activity.