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目的 观察氟乙酰胺对大鼠心肌细胞的影响及乙酰胺解毒效果。方法 将 4组SD大鼠分别给予不同剂量的氟乙酰胺 (经口灌胃 ) ,其中 2组给予治疗剂量的乙酰胺 (腹腔注射 )。观察各组中毒后不同阶段心肌细胞和心肌酶 [天冬氨酸转氨酶 (AST)、乳酸脱氢酶 (LDH)、磷酸肌酸激酶(CK)、磷酸肌酸激酶同功酶 (CK MB)、α 羟丁酸脱氢酶 (HBDH) ]的变化。结果 氟乙酰胺高剂量组 (8mg/kg)染毒后 2 4h ,AST、CK、HBDH升高 ,分别为 (5 89.5 8± 82 1.72 )、(916 .78± 343.5 5 )、(5 0 4 .4 7±14 8.88)U/L ,与对照组 [分别为 (187.70± 4 6 .87)、(75 5 .6 5± 4 98.90 )、(347.2 5± 2 2 8.4 0 )U/L]比较 ,差异有显著性 (P <0 .0 1) ;心肌细胞变性、溶解坏死和炎症细胞浸润 ,雄性大鼠 3d内全部死亡。染毒后 5d ,氟乙酰胺低剂量组 (4mg/kg)的LDH[(14 86 .5 8± 6 2 9.80 )U/L]、HBDH[(5 14 .83± 2 0 1.78)U/L]明显升高 ,与对照组 [分别为 (84 1.17± 2 18.86 )、(337.2 5± 6 2 .0 7)U/L]比较 ,差异有显著性 (P <0 .0 1)。染毒后 10d各实验组心肌酶的差异无显著性 (P >0 .0 5 ) ;病理检查仍可见一定程度的间质纤维细胞增生。给予乙酰胺 (10 0mg/kg)后可减轻病理改变的程度。结论 急性氟乙酰胺中毒可引起心肌细胞可逆
Objective To observe the effect of fluoroacetamide on rat myocardial cells and acetamide detoxification effect. Methods Four groups of SD rats were given different doses of fluoroacetamide (oral gavage), two groups were given the therapeutic dose of acetamide (intraperitoneal injection). The changes of myocardial cells and myocardial enzymes such as AST, LDH, CK, CK MB, alpha hydroxybutyrate dehydrogenase (HBDH)] changes. Results The levels of AST, CK and HBDH in the high-dose fluoroacetamide (8 mg / kg) group were (5 89.5 8 ± 82 1.72), (916.78 ± 343.5 5) and .4 7 ± 14 8.88) U / L compared with the control group [(187.70 ± 46.78), (75.56 ± 4 98.90), (347.2 ± 5 ± 22.4) U / L] The difference was significant (P <0.01). Myocardial cell degeneration, lytic necrosis and infiltration of inflammatory cells all died in 3d rats. LDH [(14 86.58 ± 62 2.80) U / L], HBDH [(5 14.83 ± 2.01.178) U / L) in low dose of fluoroacetamide group (4mg / kg) ] Was significantly higher than that of the control group [(84 1.17 ± 2 18.86) and (337.2 5 ± 6 2 .0 7) U / L], respectively. The difference was significant (P <0.01). There were no significant differences in myocardial enzymes between the experimental groups 10 days after exposure (P> 0.05). Some pathological changes of interstitial fibroblasts were still observed. Administration of acetamide (100 mg / kg) attenuated the extent of pathological changes. Conclusion Acute fluoroacetamide can cause cardiomyocyte reversible