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1978年以来,我们探索快速建成棉花黄萎病人工病圃技术。先后试用过病菌孢子悬浮液针刺注射接种(以下简称针注)、碎棉籽培养菌剂沟施接种(以下简称菌剂沟施)以及碎病秆接种等,有单项接种也有两项接种并用。以菌剂沟施和针注并用的效果最好。削秆检查,病株率达到99.8%,病情指数达到56.4,基本上做到一年建成棉花黄萎病人工病圃的要求,现将探索情况介绍如下:
Since 1978, we have explored the rapid construction of an artificial disease nursery for Verticillium dahliae. Has tried the germ spore suspension acupuncture injection (hereinafter referred to as the needle injection), broken cottonseed cultivation agent ditch vaccination (hereinafter referred to as agent ditch) and broken stalk inoculation, there are two single inoculation and vaccination. Ditch with facilities and needle injection and use the best results. Cut stalk inspection, the diseased plant rate reached 99.8%, the disease index reached 56.4, basically one year to build the artificial wilt of cotton Verticillium wilt requirements are to explore the situation are as follows: