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研究表明,蜡蚧轮枝菌(Verticillium lecanii(Zimm.))在2种液体和5种固体培养基中发酵比较,以麦芽汁液体和小米粒固体培养基中的产孢量和分生孢子活力较高。液体发酵5～12 d 的代谢产物粗提物(稀释10倍)对温室白粉虱成虫的毒杀作用,以发酵7 d 的为最好,24 h 校正死亡率为50.18%,但与发酵5 d 的(34.18%)无显著差异(P<0.05)。代谢产物粗提物对棉铃虫、麦长管蚜的触杀作用较好,对瓜蚜次之,对桃蚜最差。如试验24 h,稀释5倍的代谢产物杀虫校正死亡率,麦长管蚜66.7%、棉铃虫66.7%、瓜蚜48.8%、桃蚜23.4%。稀释2倍时,24 h 瓜蚜的校正死亡率为78.8%,略高于氰戊菊酯(7768mg·L~(-1))的毒杀作用(77.2%)。说明该菌代谢产物中具有杀虫活性物质,值得进一步研究利用。
The results showed that Verticillium lecanii (Zimm.) Was fermented in two kinds of liquid and five kinds of solid medium. The spore yield and conidia activity of the liquid medium and the millet solid medium Higher. The toxic effects of 5 ~ 12 d crude metabolites (diluted 10-fold) on adults of B. tabaci were the best at 7 d after fermentation. The corrected mortality at 24 h was 50.18% (34.18%) had no significant difference (P <0.05). Crude extracts of metabolites of cotton bollworm, wheat aphid contact role is good, followed by the melon aphid, the worst peach aphid. If tested 24 h, the metabolites diluted 5-fold insecticide-corrected mortality, wheat aphid 66.7%, cotton bollworm 66.7%, aphid 48.8%, peach aphid 23.4%. At 2-fold dilution, the corrected mortality of A. gossypii at 24 h was 78.8%, slightly higher than that of fenvalerate (77.2%). Indicating that the metabolites of the bacteria insecticidal substances, it is worth further study.