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１９９６年，胜利油田微生物采油技术研究发展中心在开展微生物采油的室内试验和矿场研究中，取得突破性进展。在室内试验研究中，筛选、培养了适应于微生物采油的厌氧和兼性厌氧菌种８０余株，分Ｆ、Ｂ两个系列，各１２组，并具有年生产６００ｔ以上菌液的能力，菌液中的细菌密度达到１０１０～１２Ｃｅｌ／Ｌ。在地层渗透率高于３０×１０－３μｍ２、地层矿化度低于１５００００ｍｇ／Ｌ、地层温度低于１２０℃、原油粘度低于４０００ｍＰａ·ｓ、含腊量高于３％的井中，用微生物进行吞吐处理均见到效果。１９９６年５月到年底，该中心先后在胜利、中原、滇黔桂等油田，矿场试验施工６０余口井、２３０多井次，有效率达７０％以上，累计净增原油８５００多吨，取得很好的经济效益。因此，这项技术具有广阔的应用前景。
In 1996, the Shengli Oilfield Microbial Production Technology Research and Development Center made breakthrough progress in the field experiment and mine research of carrying out microbial recovery. In laboratory experiments, more than 80 strains of anaerobic and facultative anaerobic bacteria were selected and cultivated, which were divided into F and B series, each of 12 groups and had the ability to produce more than 600t of bacterial liquid per year , Bacterial density in bacteria reached 1010 ~ 12Cel / L. With the formation permeability higher than 30 × 10-3μm2, the formation salinity is less than 150000mg / L, the formation temperature is lower than 120 ℃, the viscosity of the crude oil is lower than 4000mPa · s and the wax content is higher than 3% Huff handling all see the effect. From May 1996 to the end of 1996, the center successfully tested over 60 wells and more than 230 wells in fields such as Victory, Central Plains, Yunnan, Guizhou and Guangxi. The effective rate reached more than 70%, accumulatively increasing over 8,500 tons of crude oil, Achieve good economic benefits. Therefore, this technology has broad application prospects.