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将含有两个35S启动子和4个串联重复的35S增强子的Ac/Ds双元转座子标签载体转入水稻(Oryzasativa L.)基因组。100个转化植株的分析结果表明,Ds在T0植株的体细胞中切离频率高达60%。20个T1株系的600个T1植株的PCR分析结果表明,14个株系(70%)表现出较高的切离频率(约43%~100%)。Southern杂交发现在同一T1株系的不同植株中Ds转座既有相同的转座位点(约90%),也有不同的转座位点(约10%)。
Ac / Ds binary transposon tag vector containing two 35S promoters and four 35S enhancers in tandem repeats was transformed into the rice (Oryzasativa L.) genome. The analysis of 100 transformed plants showed that the frequency of Ds cleavage in somatic cells of T0 plants was as high as 60%. PCR analysis of 600 T1 plants from 20 T1 lines showed that 14 lines (70%) showed a higher cut-off frequency (about 43% -100%). Southern hybridization revealed that Ds transposon had the same transposon site (about 90%) and different transposon sites (about 10%) in different plants of the same T1 line.