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目的:研究香椿子正丁醇提取物的抗血栓作用及其机制。方法:制作大鼠静脉血栓模型测定静脉血栓的湿质量、干质量及血栓抑制率检测香椿子正丁醇提取物对体内静脉血栓形成的抑制作用;用流式细胞仪的方法检测香椿子正丁醇提取物对凝血酶引起的血小板细胞内钙离子浓度上升的抑制作用和对血小板膜GPⅡb/Ⅲa受体表达的拮抗作用,了解香椿子正丁醇提取物抗血栓作用的机制。结果:预先给予香椿子正丁醇提取物5、10、20mg/kg,能显著降低大鼠静脉血栓的湿质量和干质量;在体外实验中,药物0.12、0.24、0.48mg/ml可剂量依赖性地抑制凝血酶诱导的血小板内游离钙离子浓度的升高,尤其0.24mg/ml和0.48mg/ml组的作用更为明显;在3.84mg/ml剂量时,能降低血小板膜GPⅡb/Ⅲa受体的表达。
Objective: To study the antithrombotic effects and mechanism of n-butanol extract of Toona sinensis. METHODS: The rat venous thrombosis model was established to measure the wet weight, dry weight and thrombosis inhibition rate of venous thrombosis. The inhibitory effect of n-butanol extract of Toona sinensis on venous thrombosis in vivo was detected. The inhibitory effect of alcohol extracts on thrombin-induced increase of intracellular calcium concentration in platelets and the antagonism of platelet membrane GPIIb/IIIa receptor expression were used to understand the mechanism of the antithrombotic effects of n-butanol extract of Toona sinensis. RESULTS: Pretreatment with n-butanol extracts of 5, 10, 20 mg/kg could significantly reduce the wet and dry mass of venous thrombosis in rats. In vitro, the doses of 0.12, 0.24, and 0.48 mg/ml could be dose dependent. Suppression of thrombin-induced increase in intracellular free calcium concentration in platelets, especially in the 0.24 mg/ml and 0.48 mg/ml groups, is more pronounced; at a dose of 3.84 mg/ml, it can reduce platelet membrane GPIIb/IIIa exposure. Body expression.