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目的观察糖尿病大鼠椎间盘细胞凋亡情况,探讨可能与椎间盘细胞凋亡有关的因素。方法36只雄性SD大鼠随机分为实验组和对照组,每组18只。实验组采用腹腔单次注射链脲佐菌素溶液(streptozotocin,STZ)40mg/kg制成糖尿病模型。正常对照组仅注射等量的柠檬酸缓冲液。分别在第1、3、4个月时取腰椎间盘组织,采用流式细胞术检测椎间盘细胞凋亡率,用高效液相层析法测定椎间盘组织中戊糖甙素含量的变化。结果实验组大鼠在注射STZ溶液后,血糖水平均高于16.7mmol/L[(23.71±2.69)mmol/L]。在3个不同时期,实验组大鼠椎间盘细胞凋亡率明显高于同期对照组(P<0.05),实验组大鼠椎间盘组织中戊糖甙素含量明显高于同期对照组(P<0.05)。椎间盘组织中戊糖甙素含量与椎间盘细胞凋亡率之间呈正相关性(r=0.857～0.970,P<0.05)。结论糖尿病大鼠椎间盘细胞凋亡率明显增加。戊糖甙素等糖基化终末产物(advancedglycationendproducts,AGEs)可能是促进糖尿病大鼠椎间盘细胞凋亡的因素之一。
Objective To observe the apoptosis of rat intervertebral disc in diabetic rats and to explore the possible factors that may contribute to the apoptosis of intervertebral disc. Methods Thirty-six male SD rats were randomly divided into experimental group and control group, with 18 rats in each group. The experimental group was given a single intraperitoneal injection of streptozotocin (streptozotocin, STZ) 40mg / kg into the diabetic model. The normal control group was injected only with the same amount of citrate buffer. The lumbar intervertebral discs were harvested at the 1st, 3rd, 4th month respectively. The apoptotic rate of the intervertebral disc was detected by flow cytometry. The pentosidine content in the disc tissue was determined by HPLC. Results After injection of STZ solution, the blood glucose level in the experimental group was higher than 16.7 mmol / L [(23.71 ± 2.69) mmol / L]. The apoptosis rate of intervertebral disc in the experimental group was significantly higher than that of the control group in three different periods (P <0.05). The pentoside content in the intervertebral disc tissue of the experimental group was significantly higher than that of the control group (P <0.05) . The intervertebral disc tissue pentoside content and the rate of intervertebral disc apoptosis was positively correlated (r = 0.857 ~ 0.970, P <0.05). Conclusion The apoptosis rate of intervertebral disc in diabetic rats increased significantly. Advanced glycosylation end products (AGEs), such as pentoside, may be one of the factors that promote the apoptosis of the disc cells in diabetic rats.