论文部分内容阅读
目的 :观察伴刀豆球蛋白A是否能有效的区分出正常与肝细胞肝癌特异γ 谷氨酰转移酶 (HCC SGGT) ,为进一步建立简便、快速、灵敏、特异的检测肝癌特异GGT的方法奠定基础。方法 :用植物凝集素亲和微柱法 ,二步洗脱 ,分离 10例正常人、5例肝细胞肝癌病人、5例良性肝病病人血清中的GGT ,用速率法检测酶活性。得到层析图谱、总酶活性比和亲和率。结果 :正常与良性肝病病人血清第一步洗脱的酶活性较低(平均峰高分别为 1 62 ,1 2 6) ,而肝癌病人血清第一步洗脱的酶活性较高 (平均峰高为 16 8)。总酶活性比分别为 0 2 4,0 13 ,0 48;亲和率分别为 80 6% ,88 1% ,67 6%。结论 :伴刀豆球蛋白A与正常人、良性肝病病人血清中的GGT亲和性较强 ,与肝癌病人血清中的GGT亲和性较弱 ,伴刀豆球蛋白A能有效的区分出正常与肝癌特异GGT ,利用该特性可建立检测肝癌特异GGT的植物凝集素亲和法。
Objective: To observe whether concanavalin A can effectively distinguish between normal and hepatocellular carcinoma specific gamma glutamyl transferase (HCC SGGT), and lay a foundation for further establishing a simple, rapid, sensitive and specific method for detecting hepatoma specific GGT. basis. METHODS: GGT was separated from the serum of 10 normal subjects, 5 hepatocellular carcinoma patients and 5 patients with benign liver disease using lectin affinity microcolumn method and two-step elution. The enzyme activity was measured by the rate method. Chromatograms, total enzyme activity ratios, and affinity rates were obtained. Results: The enzyme activity of the first step in the serum of normal and benign liver disease patients was lower (average peak heights were 1 62 and 1 2 6 ), respectively, while the enzyme activity of the first step in serum of liver cancer patients was higher (average peak height). 16 8). The total enzyme activity ratios were 0 2 4, 0 13 and 0 48, respectively; the affinity rates were 80 6%, 88 1% and 67 6%, respectively. Conclusion: Concanavalin A has strong affinity with GGT in normal and benign liver disease patients, and has weaker affinity with GGT in serum of liver cancer patients. Concanavalin A can effectively distinguish normal With liver cancer-specific GGT, this property can be used to establish a lectin affinity method for detecting liver cancer-specific GGT.