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研究了甲基莲心碱（Ｎｅｆ）对氧自由基介导离体大鼠冠状血管阻力变化的影响．结果发现电解性氧自由基可引起冠脉灌注压（ＣＡＰＰ）增加；去冠状血管内皮后，电解所致ＣＡＰＰ的增加明显减弱．预先给予Ｌ－精氨酸（１００μｍｏｌ·Ｌ－１）能明显对抗氧自由基所致的ＣＡＰＰ增加，且Ｌ－精氨酸的作用能部分被硝基－Ｌ－精氨酸（２００μｍｏｌ·Ｌ－１）所对抗，提示电解所致ＣＡＰＰ的增加与氧自由基影响冠状血管内皮功能有关．Ｎｅｆ依剂量性地对抗电解所致ＣＡＰＰ的增加，且Ｎｅｆ（１０μｍｏｌ·Ｌ－１）的作用能被硝基－Ｌ－精氨酸（２００μｍｏｌ·Ｌ－１）或吲哚美辛（１μｍｏｌ·Ｌ－１）所对抗，提示Ｎｅｆ的作用可能部分通过影响前列腺素及内皮源性舒张因子类物质的代谢有关．
The effect of Nef on the oxygen free radical-mediated changes of coronary vascular resistance in isolated rats was studied. The results showed that electrolytic oxygen free radicals can cause increased coronary perfusion pressure (CAPP); to the coronary endothelium, electrolysis caused CAPP decreased significantly. Pretreatment with L-arginine (100μmol·L-1) significantly inhibited the increase of CAPP induced by oxygen free radicals, and the effect of L-arginine was partially blocked by nitro-L-arginine (200μmol·L- 1) the confrontation, suggesting that increased CAPP caused by electrolysis and oxygen free radicals affect coronary endothelial function. Nef dose-wise counteract the increase of CAPP induced by electrolysis, and the effect of Nef (10μmol·L-1) was inhibited by nitric-L-arginine (200μmol·L-1) or indomethacin -1), suggesting that the role of Nef may be partly related to the metabolism of prostaglandins and endothelium-derived relaxing substances.